Multiplex Feline Stomatitis Pathogens real time PCR detection kit(FHV/FCV/FeLV/FIV) nucleic acid detection kit

Intend for Use:

This kit is suitable for the specific detection of feline herpes virus (FHV), feline calicivirus (FCV), feline leukaemia virus (FeLV) and feline AIDS virus (FIV) in cat eye, nose and mouth secretions and EDTA whole blood samples.

Principle:

Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose cat herpes virus, cat goblet virus, feline leukemia virus, HIV gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, In this way, the 3 '-end quenched group is separated, and the fluorescence is emitted, and the nucleic acid detection of feline herpes virus, feline calicivirus, feline leukaemia virus and feline AIDS virus is realized in the completely closed reaction system by fluorescence PCR instrument.

Component:

1. Sample type

Feline eye nasal and mouth secretion and EDTA whole blood samples

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

PCR amplification and fluorescence detection (PCR detection area):

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM and VIC/HEX were selected for detection channel.

FHV/FCV PCR Reaction reagent:

Note: "+" means positive, "-" means negative

FeLV/FIV PCR PCR Reaction reagent:

Note: "+" means positive, "-" means negative

Product picture:

Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team.

The company's core team has been in the field of IVD research and entrepreneurship for more than 10 years, and has mastered a wealth of molecular diagnostic POCT industrial application technology, SmartTRF immune detection technology, RT qPCR nucleic acid detection technology and independent research and development capabilities of instruments.

It has a number of independent core technologies in the fields of automation engineering, micro-precision manufacturing, immunofluorescence, nucleic acid detection and gene sequencing. Since its establishment, the company has applied for more than 60 patents and currently has 17 authorized patents. The company has two R&D centers for diagnostic reagents and instruments and equipment, totaling more than 500 square meters; it has established more than 2,000 square meters of GMP workshops and equipment production plants; it has SmartTRF new immune detection technology, nucleic acid detection technology (POCT) and three scientific services.