Molecular Diagnosis Quantitative Multiple Feline Digestive
Pathogens Nuclic Acid detection Kit
Intend for Use:
This kit is suitable for the specific detection of feline
parvovirus (FPV), feline coronavirus (FCoV), fetal trichomonas (TF)
and giardia (GD) in cat eye, mouth and nose secretions and other
samples.
Principle:
Under the action of high-efficiency reverse transcriptase, cDNA
strands complementary to RNA template were synthesized with PRIMER
as starting point and RNA template as template. Choose cat tiny
virus, coronavirus, SAN MAO trichomonas fetus, jia drops of giardia
conservative design specific gene probe, the probe can with primer
amplification area in the middle of a specific binding DNA
template, in the process of PCR extension, the circumscribed Taq
polymerase enzymes from the 5 'end of fluorescent groups on the
probe will be cut down, make it free in the reaction system, Thus,
the 3 '-end quenched group was separated, and the fluorescence was
emitted, and the nucleic acids of feline parvovirus, feline
coronavirus, fetal trichomonas and giardia were detected by
fluorescence PCR instrument in the completely closed reaction
system.
Component:
| Composition | Quantity |
| FPV/FCoV PCR liquid | 8 tube |
| TF/GD PCR liquid | 8 tube |
| Positive control | 1 tube |
| Negative control | 1tube |
Sample requirements:
1. Sample type
Cat eye, mouth and nose secretion and other samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃.
FPV/FCoV PCR Reaction reagent:
| FAM channel (FPV) | VIC/HEX channel(FCoV) | Result interpretation |
| 1 | - | - | Results were reported negative |
| 2 | + | - | Results were reported FPV positive |
| 3 | - | + | Results were reported FCoV positive |
| 4 | + | + | Results were reported FPV,FCoV positive |
Note: "+" means positive, "-" means negative
TF/GD PCR PCR Reaction reagent:
| FAM channel (TF) | VIC/HEX channel(GD) | Result interpretation |
| 1 | - | - | Results were reported negative |
| 2 | + | - | Results were reported TF positive |
| 3 | - | + | Results were reported GD positive |
| 4 | + | + | Results were reported TF,GD positive |
Note: "+" means positive, "-" means negative
Product picture:
