Sign In | Join Free | My himfr.com
Home > Feline Cat Test Kit >

RT QPCR Feline Infectious Peritonitis Test Taq DNA Feline Coronavirus Test Kit

Guangzhou BioKey Healthy Technology Co.Ltd
Trust Seal
Verified Supplier
Credit Check
Supplier Assessment
Contact Now
    Buy cheap RT QPCR Feline Infectious Peritonitis Test Taq DNA Feline Coronavirus Test Kit from wholesalers
     
    Buy cheap RT QPCR Feline Infectious Peritonitis Test Taq DNA Feline Coronavirus Test Kit from wholesalers
    • Buy cheap RT QPCR Feline Infectious Peritonitis Test Taq DNA Feline Coronavirus Test Kit from wholesalers

    RT QPCR Feline Infectious Peritonitis Test Taq DNA Feline Coronavirus Test Kit

    Ask Lasest Price
    Brand Name : Biokey
    Model Number : BIK-Q-P029
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
    • Product Details
    • Company Profile

    RT QPCR Feline Infectious Peritonitis Test Taq DNA Feline Coronavirus Test Kit

    Feline infectious Peritonitis / corona virus RT qPCR test kit , Taq DNA polymerase probe kit



    Intended use:


    This kit is suitable for the specific detection of feline infectious peritonitis virus (FIPV) and feline coronavirus (FCoV) in cats pleural and abdominal fluid, feces, cerebrospinal fluid and other samples.


    Principle:


    Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Selects the feline infectious peritonitis and cat goblet viruses conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of feline infectious peritonitis virus and feline calicivirus can be detected by fluorescence PCR instrument in the completely closed reaction system.


    Main components:

    ComponentSpecification
    PCR reaction liquid8 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.


    Sample requirements:

    1. Sample type

    Samples of pleural and abdominal fluid, feces, cerebrospinal fluid, etc

    2. Sample collection and transportation

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.


    Using Micgene 242/244/244IVD and ABI QuantStudio5:

    PCR amplification and fluorescence Detection (PCR detection area)

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


    StepCycle numberTemperatureTime
    1150℃20min
    2195℃2min30s
    34595℃10s
    60℃30s collect fluorescent

    FAM and VIC/HEX are selected for detection channels.


    The results of interpretation

    Result interpretation


    FAM channel (FIPV)VIC/HEX channel (FCoV)Result interpretation
    1--Results were reported negative
    2+-Results were reported FIPV positive
    3-+Results were reported FCoV positive
    4++Results were reported FIPV,FCoV positive

    Note: "+" means positive, "-" means negative


    Positive judgment value or reference interval


    ResultCt Value
    PositiveCt≤38
    NegativeNo Ct value or Ct value=0
    Gray area38<Ct≤45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    Operation details please check from IFU!

    Quality RT QPCR Feline Infectious Peritonitis Test Taq DNA Feline Coronavirus Test Kit for sale
    Inquiry Cart 0
    Send your message to this supplier
     
    *From:
    *To: Guangzhou BioKey Healthy Technology Co.Ltd
    *Subject:
    *Message:
    Characters Remaining: (0/3000)