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FPV Feline Panleukopenia Test Kit PCR FCoV Test Kit Real Time

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap FPV Feline Panleukopenia Test Kit PCR FCoV Test Kit Real Time from wholesalers
     
    Buy cheap FPV Feline Panleukopenia Test Kit PCR FCoV Test Kit Real Time from wholesalers
    • Buy cheap FPV Feline Panleukopenia Test Kit PCR FCoV Test Kit Real Time from wholesalers

    FPV Feline Panleukopenia Test Kit PCR FCoV Test Kit Real Time

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    Brand Name : Biokey
    Model Number : BIK-Q-P031
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    FPV Feline Panleukopenia Test Kit PCR FCoV Test Kit Real Time

    Feline Parvovirus/Feline Coronavirus (FPV/FCoV) Dection kit

    (Real time PCR Method)


    Intended use:


    This kit is suitable for the specific detection of feline parvovirus (FPV) and feline coronavirus (FCoV) in cat feces samples.


    Principle:


    Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose feline parvovirus and coronavirus conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of feline parvovirus and feline coronavirus can be detected by fluorescence PCR instrument in the totally closed reaction system.


    Main components:

    ComponentSpecification
    PCR reaction liquid8 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.


    Sample requirements:

    1. Sample type

    Cat faeces samples


    2. Sample collection and transportation

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.


    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.


    Using Micgene 242/244/244IVD and ABI QuantStudio5:

    PCR amplification and fluorescence Detection (PCR detection area)

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


    StepCycle numberTemperatureTime
    1150℃20min
    2195℃2min30s
    34595℃10s
    60℃30s collect fluorescent

    FAM and VIC/HEX are selected for detection channels.


    The results of interpretation


    FAM channel (FPV)VIC/HEX channel (FCoV)Result interpretation
    1--Results were reported negative
    2+-Results were reported FPV positive
    3-+Results were reported FCoV positive
    4++Results were reported FPV,FCoV positive

    Note: "+" means positive, "-" means negative


    Positive judgment value or reference interval


    ResultCt Value
    PositiveCt≤38
    NegativeNo Ct value or Ct value=0
    Gray area38<Ct≤45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    Operation details please check from IFU!

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