Pig Pseudorabies Virus(gB gene) (PRV-gB) Detection Kit , DNA taq
polymerase qPCR Probe kit
(PCR-Fluorescent Probe Method)
Intended use:
This kit is used for the diagnosis, monitoring and epidemiological
investigation of Pseudorabies Virus(gB gene), and is suitable for
the specific detection of PRV-GB gene in pig serum, feces, semen,
viscera and other tissue samples.
Principle:
This kit use pig Pseudorabies Virus(gB gene) conservative design
specific gene probe, the probe can with primer amplification area
in the middle of a specific binding DNA template, in the process of
PCR extension, the circumscribed Taq polymerase enzymes from the 5
'end of fluorescent groups on the probe will be cut down, make it
free in the reaction system, thereby quenching groups out of the 3'
end, Fluorescence was generated and detected by fluorescence PCR
instrument to realize nucleic acid detection of PORcine
PSEUDorabies virus gB gene in the completely closed reaction
system.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type:
Pig serum, feces, semen, viscera and other tissue samples
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
