Porcine circovirus type 2 (PCV2) Nucleic Acid Detection Kits
(PCR-Fluorescent Probe Method)
Intended use:
This kit is used for diagnosis, monitoring and epidemiological
investigation of porcine circovirus type 2, and is suitable for
specific detection of porcine circovirus type 2 (PCV2) in pig
serum, feces, semen, viscera and other tissue samples.
Principle:
This kit selection of porcine circovirus virus type 2 conservative
design specific gene probe, the probe can with primer amplification
area in the middle of a specific binding DNA template, in the
process of PCR extension, the circumscribed Taq polymerase enzymes
from the 5 'end of fluorescent groups on the probe will be cut
down, make it free in the reaction system, thereby quenching groups
out of the 3' end, The porcine circovirus type 2 nucleic acid was
detected by fluorescence PCR instrument in a completely closed
reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type:
Pig serum, feces, semen, viscera and other tissue samples
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
