Swine transmissible gastroenteritis virus(TGEV)Nucleic Acid
Detection Kit (RT-PCR-Fluorescent Probe Method)
Intended use:
This kit is suitable for Swine transmissible gastroenteritis
virus(TGEV)in small intestine and fresh feces of pigs.
Principle:
This kit uses RNA as a template and primers as a starting point to
synthesize cDNA strands complementary to RNA
template under the action of high-efficiency reverse transcriptase.
Choose the pig epidemic diarrhea virus gene
conservative design specific probe, the probe with primer
amplification area in the middle of a specific binding DNA
template, in the process of PCR extension, the circumscribed Taq
polymerase enzymes from the 5 'end of fluorescent
groups on the probe will be cut down, make it free in the reaction
system, thereby quenching groups out of the 3' end,
glow, The detection of Swine transmissible gastroenteritis
virus(TGEV) in a completely closed reaction system was
realized by fluorescence PCR.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 40＜Ct<45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is
considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy:
The positive reference materials of testing enterprises were all
positive.
2. Minimum detection limit:
The detection limit of this kit is 3 copies/μL.
3. Precision:
Coefficient of variation (CV,%) of intra-batch precision Ct value
is ≤5%.
4.Linearity:
Linear correlation coefficient| r | >0.980
