Porcine reproductive and respiratory disorders syndrome(CN) Nucleic
Acid Detection Kit (PCR-Fluorescent Probe Method)
Intended use:
This kit is used for the diagnosis, monitoring and epidemiological
investigation of porcine blue ear disease, and is suitable for the
specific detection of Porcine Reproductive and respiratory syndrome
virus-Chinese strain/highly pathogenic (PRRSV-CN) in pig serum,
oral swab, lung lavage fluid, semen, tissue (lung, lymph node,
tonsil, spleen, etc.).
Principle:
This kit uses RNA as a template and primers as a starting point to
synthesize cDNA strands complementary to RNA template under the
action of high-efficiency reverse transcriptase. Selection of
porcine reproductive and respiratory syndrome virus - China/highly
pathogenic strains conservative design specific gene probe, the
probe can with primer amplification area in the middle of a
specific binding DNA template, in the process of PCR extension, the
circumscribed Taq polymerase enzymes from the 5 'end of fluorescent
groups on the probe will be cut down, make it free in the reaction
system, Thus, the 3 '-end quenched group was separated, and
fluorescence was emitted, which was detected by fluorescence PCR
instrument to realize the detection of Porcine reproductive and
respiratory syndrome virus-Chinese strain/highly pathogenic nucleic
acid in a completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type
Pig serum, oral swab, lung lavage fluid, semen, tissue (lung, lymph
node, tonsil, spleen, etc.) and other samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃.
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all
positive.
2. Minimum detection limit
The detection limit of this kit is 3 copies/μL.
3. Precision
Coefficient of variation (CV,%) of intra-batch precision Ct value
is ≤5%.
