Veterinary Feline leukemia virus/Feline AIDS virus (FeLV/FIV) DNA
taq polymerase probe qPCR kit
Intended use:
This kit is suitable for the specific detection of feline leukemia
virus (FeLV) and feline AIDS virus (FIV) in EDTA whole blood
sample.
Principle:
Under the action of high-efficiency reverse transcriptase, cDNA
strands complementary to RNA template were synthesized with PRIMER
as starting point and RNA template as template. Choose feline
leukemia virus (FeLV) and feline AIDS virus (FIV) gene conservative
design specific probe, the probe can with primer amplification area
in the middle of a specific binding DNA template, in the process of
PCR extension, the circumscribed Taq polymerase enzymes from the 5
'end of fluorescent groups on the probe will be cut down, make it
free in the reaction system, thereby quenching groups out of the 3'
end, Fluorescence was emitted and detected by fluorescence PCR
instrument to realize nucleic acid detection of f feline leukemia
virus (FeLV) and feline AIDS virus (FIV) virus in the completely
closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 8 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Product pictures:
Sample requirements:
1. Sample type
EDTA whole blood
2. Sample collection and transportation
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃.
PCR amplification and fluorescence Detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR
amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 50℃ | 20min |
| 2 | 1 | 95℃ | 2min30s |
| 3 | 45 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
FAM and VIC/HEX are selected for detection channels.
The results of interpretation:
| FAM channel (FeLV) | VIC/HEX channel (FIV) | Result interpretation |
| 1 | - | - | Results were repoFIVrted negative |
| 2 | + | - | Results were reported FeL positive |
| 3 | - | + | Results were reported FIV positive |
| 4 | + | + | Results were reported FeLV,FIV positive |
Note: "+" means positive, "-" means negative
Positive judgment value or reference interval:
| Result | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 38＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
Operation details please check from IFU!
