Epstein-Barr virus (EBV) real-time PCR detection kit (lyophilized)
--96 tests/kit
Intended use:
This kit, adopting Real-Time PCR-Fluorescence technology, is used
for detection of Epstein-Barr virus (EBV). It can be used for the
laboratory diagnosis and monitoring of EBV infection. The test
results are only for clinical reference, not for case confirmation
or exclusion.
Principle:
The specific primers and specific fluorescent probes are designed
for the nucleic acid conserved region of Epstein-Barr virus (EBV).
The PCR reaction solution and real-time fluorescent quantitative
PCR detection technology is applied on the fluorescence
quantitative PCR instrument to realize the rapid detection of EBV
through the change of fluorescence signal. The detection system
contains a human internal control (IC) gene, which is used to
monitor whether the specimens and the process of nucleic acid
extraction is qualified.
Main components:
| No. | Components | BIK-QL-H0017 |
1 | PCR Enzyme Mix (Lyophilized) | 96 tests/bottle |
| 2 | EBV Primer-Probe Mix | 100 μL/vial |
| 3 | Enzyme Mix Buffer (5×) | 400 μL/vial |
| 4 | Positive Control | 100 μL/tube |
| 5 | Negative Control | 100 μL/tube |
| 6 | RNase-free water | 1mL/tube |
| 7 | Paraffin oil | 1.5mL/tube*2 |
Note: Do not mix the components from different batches for
detection. The Positive Control of EBV and internal control were
constructed artificially, and they were not infectious.
Specimen requirements:
1.Specimen type :
Whole blood, serum or plasma.
2.Specimen preservation:
The specimen can be stored for 3 months at -20±5℃ and for a long
time below -70℃
Reaction mix preparation:
Refer to the tables below to prepare the reaction mix:
| 1×volume required |
| Resuspended master mix | 14 μL |
EBV Primer & probe | 1 μL |
| Total volume | 15 μL |
※Multiply the numbers according to the number of tests.
Quality control:
Negative Control, Positive Control and IC of samples must be
performed correctly, otherwise the sample results is invalid.
| Ct value |
| Negative Control | No Ct |
| Positive Control | ≤30 |
| IC of samples | ≤38 |
PCR amplification condition:
PCR amplification according to the following procedure:
| Step | Cycle | Temperature | Time |
| 1 | 1 | 95℃ | 3 min |
2 |
40 | 95℃ | 10 s |
60℃ | 30 s (Collection of fluorescence) |
Instrument detection channel selection: FAM and HEX/VIC.
Operation details please check from IFU!
