Sign In | Join Free | My himfr.com
Home > Aquaculture Test Kit >

Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection

Guangzhou BioKey Healthy Technology Co.Ltd
Trust Seal
Verified Supplier
Credit Check
Supplier Assessment
Contact Now
    Buy cheap Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection from wholesalers
     
    Buy cheap Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection from wholesalers
    • Buy cheap Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection from wholesalers
    • Buy cheap Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection from wholesalers
    • Buy cheap Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection from wholesalers

    Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection

    Ask Lasest Price
    Brand Name : Biokey
    Model Number : BIK-Q-W021
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
    • Product Details
    • Company Profile

    Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection

    Real Time Fluorescence qPCR Detection System Bacterial / Vibrio Count (Bac/ Vib) PCR test Kit


    Intended use:


    This kit is used for real-time detection of bacteria and vibrio by fluorescence probe PCR method, and is suitable for rapid quantitative detection of bacteria and vibrio by FAM fluorescence channel and HEX fluorescence channel.


    Principle:

    This kit to choose total bacteria/vibrio specific fragment probes the design of primers, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The total number of bacteria/vibrio nucleic acid in the total closed reaction system can be detected by fluorescence PCR instrument.​

    Main components:

    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.

    Sample requirements:
    1. Sample type

    Aquaculture water, prawns, etc.

    2. Transport

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

    PCR amplification and fluorescence detection (PCR detection area):

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


    StepCycle numberTemperatureTime
    1155℃5min
    2194℃5min
    34094℃10s
    60℃25s collect fluorescent

    FAM and HEX/VIC were selected for detection channels

    Product picture:



    Product performance index:

    1. Accuracy

    The positive reference materials of testing enterprises were all positive.

    2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

    3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.


    Quality control:


    (1) Quality control of total bacterial count detection in FAM fluorescence channel:

    Negative control: CT value should be ≥29, positive control: CT value should be ≤25.

    (2)HEX/VIC fluorescent channel vibrio count detection quality control:

    Negative control: CT value should be ≥35, positive control: CT value should be ≤30.


    Operation details please check from IFU!

    Quality Real Time Pcr Bacteria Test Kit Vibrio Test Kit HEX Fluorescence QPCR Detection for sale
    Inquiry Cart 0
    Send your message to this supplier
     
    *From:
    *To: Guangzhou BioKey Healthy Technology Co.Ltd
    *Subject:
    *Message:
    Characters Remaining: (0/3000)