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Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit from wholesalers
     
    Buy cheap Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit from wholesalers
    • Buy cheap Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit from wholesalers
    • Buy cheap Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit from wholesalers

    Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit

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    Brand Name : Biokey
    Model Number : BIK-Q-W008
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit

    Shrimp Hemocyte Iridescent Virus ( SHIV ) Nucleic Acid Primer Probe PCR Detection Kit



    Intended use:


    This kit is suitable for the detection of Shrimp Hemocyte Iridescent Virus (SHIV) infection in adult prawn, prawn parent, prawn seedling, bait, processed aquatic products, etc.

    Principle:


    This kit uses a specific fragment of Shrimp Hemocyte Iridescent Virus (SHIV) to design a primer probe, which can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cuts the 5 '-terminal fluorescein from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of Shrimp Hemocyte Iridescent Virus (SHIV) was detected in the completely closed reaction system.


    Main components:

    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.


    Sample requirements:

    1. Sample type

    Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

    2. Transport

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.


    Using Micgene 242/244/244IVD and ABI QuantStudio5:

    PCR amplification and fluorescence Detection (PCR detection area)

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


    StepCycle numberTemperatureTime
    1195℃3min
    24595℃10s
    60℃30s collect fluorescent

    FAM is selected for detection channels.


    The results of interpretation:


    ResultsCt Value
    PositiveCt≤45
    NegativeNo Ct value or Ct value≥45
    Gray area40<Ct≤45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    Operation details please check from IFU!

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