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Brand Name : | Biokey |
Model Number : | BIK-Q-W026 |
Certification : | ISO 13485 |
Price : | USD |
Payment Terms : | L/C, T/T, Western Union |
Supply Ability : | 100,000 per day |
Delivery Time : | Depending on order quantity |
Channel Catfish Virus Septicaemia Virus ( CCV ) Nucleic Acid DNA Amplification qPCR Kit
Intended use:
This kit is suitable for Channel catfish virus septicaemia virus
(CCV) of liver, spleen and kidney tissue samples.
Principle:
This kit selection Channel catfish virus septicaemia virus (CCV)
specific fragment to design primers probe probe, the probe can with
primer amplification area in the middle of a specific binding DNA
template, in the process of PCR extension, the circumscribed Taq
polymerase enzymes from the 5 'end of fluorescent groups on the
probe will be cut down, make it free in the reaction system,
thereby quenching groups out of the 3' end, glow, Fluorescence PCR
instrument was used to detect Channel catfish virus septicaemia
virus CCV in a completely closed reaction system.
Main components:
Component | Specification |
PCR reaction liquid | 24 tubes |
Positive control | 1 tube |
Negative control | 1 tube |
1. Sample type
Channel catfish”Liver, spleen, kidney, etc
2.Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T
7103. Samples shall be transported to the laboratory as soon as
possible under refrigerated conditions to avoid repeated
freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 95℃ | 3min |
2 | 45 | 95℃ | 10s |
60℃ | 30s fluorescent |
FAM is selected for detection channels.
The results of interpretation:
Results | Ct Value |
Positive | Ct≤38 |
Negative | No Ct value or Ct value≥45 |
Gray area | 38<Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all
positive.
2. Minimum detection limit: the detection limit of this kit is
10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch
precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
Operation details please check from IFU!
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