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Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit from wholesalers
     
    Buy cheap Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit from wholesalers
    • Buy cheap Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit from wholesalers
    • Buy cheap Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit from wholesalers

    Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit

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    Brand Name : Biokey
    Model Number : BIK-Q-W026
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit

    Channel Catfish Virus Septicaemia Virus ( CCV ) Nucleic Acid DNA Amplification qPCR Kit


    Intended use:

    This kit is suitable for Channel catfish virus septicaemia virus (CCV) of liver, spleen and kidney tissue samples.

    Principle:

    This kit selection Channel catfish virus septicaemia virus (CCV) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, Fluorescence PCR instrument was used to detect Channel catfish virus septicaemia virus CCV in a completely closed reaction system.

    Main components:

    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.
    Product picture:

    Sample requirements:

    1. Sample type
    Channel catfish”Liver, spleen, kidney, etc

    2.Sample collection and transportation
    Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
    3. Sample preservation
    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

    PCR amplification and fluorescence detection (PCR detection area):

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

    StepCycle numberTemperatureTime
    1195℃3min
    24595℃10s
    60℃30s fluorescent

    FAM is selected for detection channels.

    The results of interpretation:

    ResultsCt Value
    PositiveCt≤38
    Negative

    No Ct value or Ct value≥45

    Gray area38<Ct≤45


    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

    Product performance index:

    1. Accuracy
    The positive reference materials of testing enterprises were all positive.
    2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
    3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
    4. Linear, linear correlation coefficient | r | > 0.980.


    Operation details please check from IFU!

    Quality Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit for sale
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