Medical diagnostic Hepatopancreas Parvovirus ( HPV ) Nucleic Acid
Magnetic Beads Fluorescent Probe PCR test kit
Intended use:
This kit is suitable for auxiliary diagnosis of hepatopancreas
parvovirus (HPV) infection.
Principle:
This kit hepatopancreas parvovirus conservative design specific
gene probe, the probe can with primer amplification area in the
middle of a specific binding DNA template, in the process of PCR
extension, the circumscribed Taq polymerase enzymes from the 5 'end
of fluorescent groups on the probe will be cut down, make it free
in the reaction system, thereby quenching groups out of the 3' end,
glow, The nucleic acid of hepatopancreas parvovirus was detected by
fluorescence PCR apparatus in a completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 24 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type
Edible prawn, machijiming prawn, vannamei and other susceptible
prawn tissues
2. Sample collection
About 0.1g tissue samples were taken from prawn samples according
to different sizes or infection stages. Among them, whole
individuals were taken from larvae and larvae, head and chest of
eyes were removed from juveniles under 3CM, gill area was taken
from larger juveniles, and gill filaments or heart or lymphatic
organs were taken from adult shrimp.
3. Storage and transportation
Samples are transported in ice bags of 2℃~8℃, and repeated freezing
and thawing are prohibited. Short-term storage at -20℃, -70℃ can be
long-term storage.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR
amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 2 | 1 | 95℃ | 3min |
| 3 | 45 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
FAM is selected for detection channels.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
Operation details please check from IFU!
