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Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit from wholesalers
     
    Buy cheap Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit from wholesalers
    • Buy cheap Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit from wholesalers

    Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

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    Brand Name : Biokey
    Model Number : BIK-Q-W015
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

    Enterocytozoon Acute hepatopancreatic necrosis syndrome Early mortality syndrome/Enterocytozoon hepatopenaei-18s(EMS/EHP-18S) Nuclear double nucleic acid test kit



    Intended use:


    This kit is suitable for the specific detection of Enterocytozoon hepatopenaei Acute hepatopancreatic necrosis syndrome Early mortality syndrome /Enterocytozoon hepatopenaei-18s (AHPND/EMS) and hepatoenteric cytiworm -18S (EHP-18S) in adult shrimp, prawn shrimp, shrimp seedlings, bait, processed aquatic products, etc.


    Principle:


    This kit is chosen prawn acute liver necrosis of pancreas disease and shrimp deeply cell worm - 18 s conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenchable group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of acute hepatopancreas necrosis and hepatoenteric cytiworm -18S nucleic acid of shrimp in the completely closed reaction system.


    Main components:

    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.


    Sample requirements:

    1. Sample type

    Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products and other samples


    2. Sample collection and transportation

    Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.


    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.


    Using Micgene 242/244/244IVD and ABI QuantStudio5:

    PCR amplification and fluorescence Detection (PCR detection area)

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


    StepCycle numberTemperatureTime
    1195℃3min
    24095℃10s
    60℃30s collect fluorescent

    FAM and VIC/HEX are selected for detection channels.


    Result interpretation:


    FAM channel (EMS)VIC/HEX channel(EHP-18S)Results judgment
    1--The results were reported negative
    2+-The results were reported EMS positive
    3-+The results were reported EHP-18S positive
    4++The results were reported EMS /EHP-18S positive

    Note: "+" means positive, "-" means negative


    The results of interpretation


    ResultsCt Value
    PositiveCt≤38
    NegativeNo Ct value or Ct value=0
    Gray area38<Ct≤40

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 40 is considered as


    Operation details please check from IFU!

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