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Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit from wholesalers
     
    Buy cheap Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit from wholesalers
    • Buy cheap Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit from wholesalers
    • Buy cheap Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit from wholesalers
    • Buy cheap Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit from wholesalers

    Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit

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    Brand Name : Biokey
    Model Number : BIK-Q-W007
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Shrimp Tissues Vpa Vibrio Parahaemolyticus Real Time PCR detection Kit

    Vibrio parahaemolyticus kit Nucleic Acid PCR Detection Kit

    (PCR-Fluorescent Probe Method)

    Intended use:

    This kit is suitable for the specific detection of pathogenic vibrio parahaemolyticus (Vpa) in bait, water samples, shrimp seedlings, shrimp tissues and processed products.


    Principle:


    This kit uses a specific fragment of Vibrio parahaemolyticus (Vpa) to design a primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclide activity of Taq enzyme cuts the 5 '-terminal fluorophore from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument to achieve the detection of Vibrio parahaemolyticus nucleic acid (Vpa) in the completely closed reaction system.

    Main components:

    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.


    Sample requirements:

    1. Sample type

    Shrimp seedling, shrimp tissue, water body, bait, processed products, etc


    2. Sample collection and transportation

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.


    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.



    Using Micgene 242/244/244IVD and ABI QuantStudio5:

    procedures are set as below:

    Steps

    Cycle number

    TemperatureTime
    1195℃3min
    24595℃10s
    60℃

    30sCollect the fluorescent

    FAM was selected for detection channel.

    The results of interpretation

    ResultsCt Value
    PositiveCt≤40
    NegativeNo Ct value or Ct value≥45
    Gray area40<Ct<45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

    Result analysis:
    Automatic analysis using instrumentation

    Quality control:
    Negative control: No obvious amplification curve of FAM detection channel;
    Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

    The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.


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