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Enterocytozoon Hepatopenaei-18s Nucleic Acid Amplification Multiplex Real Time PCR

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    Buy cheap Enterocytozoon Hepatopenaei-18s Nucleic Acid Amplification Multiplex Real Time PCR from wholesalers
     
    Buy cheap Enterocytozoon Hepatopenaei-18s Nucleic Acid Amplification Multiplex Real Time PCR from wholesalers
    • Buy cheap Enterocytozoon Hepatopenaei-18s Nucleic Acid Amplification Multiplex Real Time PCR from wholesalers

    Enterocytozoon Hepatopenaei-18s Nucleic Acid Amplification Multiplex Real Time PCR

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    Brand Name : Biokey
    Model Number : BIK-Q-W005
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Enterocytozoon Hepatopenaei-18s Nucleic Acid Amplification Multiplex Real Time PCR

    Enterocytozoon hepatopenaei-18s (EHP-18S)Nucleic Acid Detection Kits

    (PCR-Fluorescent Probe Method)


    Intended use:

    This kit is suitable for the quarantine of Enterocytozoon hepatopenaei -18S (EHP-18S) before the purchase of larvae in the shrimps desalt plant to ensure that larvae do not carry the disease pathogen. Aquaculture enterprises have conducted EHP specific detection on the bottom mud of aquaculture ponds with slow growth of prawn to exclude the hidden danger of liver and intestinal cytiworm parasitization.

    Principle:

    In this kit, a primers probe was designed by using a specific fragment of Enterocytozoon hepatopenaei -18S (EHP-18S). The probe could specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cut the 5 '-terminal fluorophore from the probe and made it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the detection of eHP-18S nucleic acid was realized in the completely closed reaction system.

    Main components:

    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.


    Sample requirements:

    1. Sample type

    Seedlings: parent, shrimp eggs, seedlings, coarse standard products

    Pond: bottom mud, excrement, water body

    Bait: live bait, chilled bait and non-high-temperature treated bait samples

    2. Transport

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.



    Using Micgene 242/244/244IVD and ABI QuantStudio5:

    procedures are set as below:

    Steps

    Cycle number

    TemperatureTime
    1195℃3min
    24595℃10s
    60℃

    30sCollect the fluorescent

    FAM was selected for detection channel.

    The results of interpretation

    ResultsCt Value
    PositiveCt≤40
    NegativeNo Ct value or Ct value≥45
    Gray area40<Ct<45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

    Result analysis:
    Automatic analysis using instrumentation

    Quality control:
    Negative control: No obvious amplification curve of FAM detection channel;
    Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

    The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.


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