Infectious hypodermal and hematopoietic necrosis virus(IHHNV) Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

Intended use:

This kit is suitable for the specific detection of IHHNV in water samples, shrimp seedlings, bait, etc., and is used for the etiological diagnosis of suspected infected persons.

Principle:

This kit is chosen infectious subcutaneous and hematopoietic organ necrosis virus (IHHNV) specific fragment to design primers probe probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of infectious subcutaneous and hematopoietic necrosis virus (IHHNV) in a completely closed reaction system.

Main components:

Sample requirements:

1. Sample type

Seedlings: parent, shrimp eggs, seedlings, coarse standard products

Pond: bottom mud, excrement, water body

Bait: live bait, chilled bait and non-high-temperature treated bait samples

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

Using Micgene 242/244/244IVD and ABI QuantStudio5:

procedures are set as below:

FAM was selected for detection channel.

The results of interpretation

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

Result analysis:

Automatic analysis using instrumentation

Quality control:

Negative control: No obvious amplification curve of FAM detection channel;

Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team.

The company's core team has been in the field of IVD research and entrepreneurship for more than 10 years, and has mastered a wealth of molecular diagnostic POCT industrial application technology, SmartTRF immune detection technology, RT qPCR nucleic acid detection technology and independent research and development capabilities of instruments.

It has a number of independent core technologies in the fields of automation engineering, micro-precision manufacturing, immunofluorescence, nucleic acid detection and gene sequencing. Since its establishment, the company has applied for more than 60 patents and currently has 17 authorized patents. The company has two R&D centers for diagnostic reagents and instruments and equipment, totaling more than 500 square meters; it has established more than 2,000 square meters of GMP workshops and equipment production plants; it has SmartTRF new immune detection technology, nucleic acid detection technology (POCT) and three scientific services.