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Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit from wholesalers
     
    Buy cheap Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit from wholesalers
    • Buy cheap Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit from wholesalers

    Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit

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    Brand Name : Biokey
    Model Number : BIK-Q-W002
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit

    Infectious hypodermal and hematopoietic necrosis virus(IHHNV) Nucleic Acid Detection Kits

    (PCR-Fluorescent Probe Method)


    Intended use:


    This kit is suitable for the specific detection of IHHNV in water samples, shrimp seedlings, bait, etc., and is used for the etiological diagnosis of suspected infected persons.


    Principle:


    This kit is chosen infectious subcutaneous and hematopoietic organ necrosis virus (IHHNV) specific fragment to design primers probe probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of infectious subcutaneous and hematopoietic necrosis virus (IHHNV) in a completely closed reaction system.


    Main components:


    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.

    Sample requirements:


    1. Sample type

    Seedlings: parent, shrimp eggs, seedlings, coarse standard products

    Pond: bottom mud, excrement, water body

    Bait: live bait, chilled bait and non-high-temperature treated bait samples

    2. Transport

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.


    Using Micgene 242/244/244IVD and ABI QuantStudio5:


    procedures are set as below:

    Steps

    Cycle number

    TemperatureTime
    1195℃3min
    24595℃10s
    60℃

    30sCollect the fluorescent

    FAM was selected for detection channel.


    The results of interpretation

    ResultsCt Value
    PositiveCt≤45
    NegativeNo Ct value or Ct value≥45
    Gray area40<Ct≤45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    Result analysis:

    Automatic analysis using instrumentation


    Quality control:

    Negative control: No obvious amplification curve of FAM detection channel;

    Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;


    The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.


    Product Tags:

    IHHNV Taqman QPCR Kit

      
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