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T Foetus One Step Real Time Pcr Kit ISO 13485 Giardia Pcr Test

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap T Foetus One Step Real Time Pcr Kit ISO 13485 Giardia Pcr Test from wholesalers
     
    Buy cheap T Foetus One Step Real Time Pcr Kit ISO 13485 Giardia Pcr Test from wholesalers
    • Buy cheap T Foetus One Step Real Time Pcr Kit ISO 13485 Giardia Pcr Test from wholesalers

    T Foetus One Step Real Time Pcr Kit ISO 13485 Giardia Pcr Test

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    Brand Name : Biokey
    Model Number : BIK-Q-P032
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    T Foetus One Step Real Time Pcr Kit ISO 13485 Giardia Pcr Test

    T.foetus/Giardia(TF/GD) nucleic acid testing kit

    (Real time PCR Method)


    Intended use:


    This kit is suitable for the specific detection of T.foetus (TF) and giardia (GD) in fetal stool samples.


    Principle:


    This kit selection and the drop of the T.foetus/Giardia(TF/GD) conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acids of trichomonas and giardia in the fetus were detected in the completely closed reaction system.


    Main components:

    ComponentSpecification
    PCR reaction liquid8 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.


    Sample requirements:

    1. Sample type

    Stool samples


    2. Sample collection and transportation

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.


    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.


    Using Micgene 242/244/244IVD and ABI QuantStudio5:

    PCR amplification and fluorescence Detection (PCR detection area)

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


    StepCycle numberTemperatureTime
    1195℃3min
    24595℃10s
    60℃30s collect fluorescent

    FAM and VIC/HEX are selected for detection channels.


    The results of interpretation


    FAM channel (CF)VIC/HEX channel (MF)Result interpretation
    1--Results were reported negative
    2+-Results were reported TF positive
    3-+Results were reported GD positive
    4++Results were reported TF,GD positive

    Positive judgment value or reference interval


    ResultCt Value
    PositiveCt≤38
    NegativeNo Ct value or Ct value=0
    Gray area38<Ct≤45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    Operation details please check from IFU!

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