Quinolones lateral flow assay diagnostics rapid test card tissue
Aquatic products
Overview:
Quinolones (QNS) quick detection card is convenient, fast and
sensitive, suitable for on-site mass sample detection. The test
card contains antigen in the test area (T) and anti-ⅱ in the
control area (C) of nitrocellulose membrane fixed in advance, and
gold label antibody fixed on the binding pad. According to T area,
C area color contrast to determine the result. Regardless of the
presence of quinolones in the sample, a purplish red band appeared
in region C.
Detection limit:
Pork, chicken, duck, fish, shrimp, etc. :
(Note: PPB =µg/kg)
Well, the effect of... 40 ppb
Ciprofloxacin... 40 ppb
Norfloxacin... 40 ppb
Flumequine... 40 ppb
Maintain... 40 ppb
Of fluorine of sand... 40 ppb
Evil sinensis acid... 40 ppb
According to the north of sand... 40 ppb
Mr Fluorine effect... 40 ppb
Ma confirmed the effect of... 40 ppb
Ofloxacin... 40 ppb
Product component:
| Item No. | Name |
| 1 | Quinolones rapid test card |
| 2 | Extracting solution A |
| 3 | Extracting solution B |
| 4 | Reconstitution fluid |
| 5 | User manual |
Preservation and stability:
Store in dry place at 20±5℃, do not freeze, avoid direct sunlight.
This test card is valid for 12 months, and the production date is
shown in the packaging box.
Materials required but not provided:
| Item No. | Name |
| 1 | Homogenizer |
| 2 | Vortex Oscillator |
| 3 | Electronic Balance |
| 4 | Nitrogen (air) blower |
| 5 | Micropipette |
Detection Procedure:
1. Remove skin and fat from the samples to be tested and homogenize
them with homogenizer;
2.Weigh 1.00±0.05 g homogenized sample into 10 mL centrifuge tube;
3.0.8ml extract A was added and vortexized for 1 min;
4. Add 4 mL extract B, vortex for 3 min, and centrifuge for 5 min
at 4000 g;
5. Remove 500 µL supernatant into 5 mL centrifuge tube and blow dry
with nitrogen (air) at 60℃;
6. Add 400 µL complex solution to the blow-dried centrifugal tube,
shake well, and wait for inspection;
7. Take out the test card, place it flat on the table after
opening, and carefully absorb 100 µL of supernatant from the
centrifuge tube into the sample well with a pipette;
8. 5 min after adding samples, observe the color area and determine
the result.
Result Judgment:
Negative: C line color, T line darker than C line color or
consistent,The test results were negative.
Positive: C line is colored, T line is lighter than C line or T
line is light green, and the test result is positive.
Invalid: Line C does not display color, regardless of whether line
T displays color, the detection card is judged to be invalid.
