Ofloxacin lateral flow assay diagnostics rapid test card tissue,
Aquatic products
Overview:
Ofloxacin (OFL) rapid detection card is convenient, fast and
sensitive, suitable for on-site mass sample detection. The test
card contains an antigen in the test area (T) and an antibody ⅱ in
the control area (C) which are fixed in advance in the
nitrocellulose membrane. After the sample was dissolved, the
gold-labeled antibody in the micropores was incubated for 3 min at
room temperature, and the gold-labeled microporous solution was
added to the detection card and sample Wells for 5-8 min reaction.
According to T area, C area color contrast to determine the result.
Whether ofloxacin was present or not, a purplish red band appeared
in region C.
Detection limit:
Pork, chicken, duck, fish, and shrimp: 2 PPB (µg/kg)
Product component:
| Item No. | Name |
| 1 | Ofloxacin rapid test card |
| 2 | Jinbiao microporous |
| 3 | NaCl solution |
| 4 | Sample extract |
| 5 | n-hexane |
| 6 | Sample Reconstitution fluid |
| 7 | User manual |
Preservation and stability:
Store in dry place at 20±5℃, do not freeze, avoid direct sunlight.
This test card is valid for 12 months. The production date is shown
in the package
Materials required but not provided:
| Item No. | Name |
| 1 | Homogenizer |
| 2 | Vortex Oscillator |
| 3 | Electronic Balance |
| 4 | Nitrogen (air) blower |
| 5 | Micropipette |
| 6 | Centrifuge |
Detection Procedure:
1. Restore the test card and sample solution to room temperature
before use;
2. Open the aluminum foil bag, take the required amount of gold
label microhole box detection card, mark and put it on the table
(note: after removing the microhole, seal the aluminum foil bag
immediately to prevent moisture);
3. Absorb 120 μL of the sample to be tested into the gold label
microhole, blow carefully until the purplish red particles at the
bottom of the hole are completely dissolved (note: blow gently to
avoid bubbles), and incubate at room temperature for 3 min.
4. Add all the solution in the gold label microhole to the
detection card and sample well for reaction of 5-8 min and then
interpret the result.
Result Judgment:
Negative: C line color, T line darker than C line color or
consistent,The test results were negative.
Positive: C line is colored, T line is lighter than C line or T
line is light green, and the test result is positive.
Invalid: Line C does not display color, regardless of whether line
T displays color, the detection card is judged to be invalid.
