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Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit from wholesalers
     
    Buy cheap Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit from wholesalers
    • Buy cheap Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit from wholesalers

    Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit

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    Brand Name : Biokey
    Model Number : BIK-Q-W001
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit

    White spot syndrome virus WSSV Nucleic Acid Detection Kits

    (PCR-Fluorescent Probe Method)


    Intended use:


    This kit is suitable for the detection of Shrimp white spot syndrome virus (WSSV) in shrimp samples according to the different size or infection stage of different tissue samples, used for the auxiliary diagnosis of shrimp white spot syndrome virus (WSSV) infection.


    Principle:


    This kit uses a specific fragment of white spot syndrome virus (WSSV) to design a primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclization activity of Taq enzyme cuts the 5 '-terminal fluoromeric group from the probe, making it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of white spot syndrome virus (WSSV) was detected in the completely closed reaction system.


    Biokey Aquaculture PCR testing solution.pdf


    Main components:


    ComponentSpecification
    PCR reaction liquid24 tubes
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.

    Sample requirements:


    1. Sample type

    Tissue samples from prawn.

    2. Transport

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3.Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

    4. Sample extraction&purification processing with professional machine below:

    Using Micgene 242/244/244IVD and ABI QuantStudio5:


    procedures are set as below:

    Steps

    Cycle number

    TemperatureTime
    1195℃3min
    24595℃10s
    60℃

    30sCollect the fluorescent

    FAM was selected for detection channel.


    The results of interpretation

    ResultsCt Value
    PositiveCt≤45
    NegativeNo Ct value or Ct value≥45
    Gray area40<Ct≤45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    procedures are set as below:


    StepsCycle numberTemperatureTime
    1195℃3min
    21095℃10s
    60℃30s
    33595℃10s
    60℃30sCollect the fluorescent

    FAM was selected for detection channel.


    The results of interpretation:


    Results

    Ct Value
    PositiveCt≤35
    NegativeNo Ct value or Ct value≥35
    Gray area30<Ct<35

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 35 is considered as positive, and no Ct value or Ct value ≥35 is considered as negative.


    Details please check from IFU!


    We offer the full PCR testing solution to support the shrimp disease detection and monitoring on site.


    Quality Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit for sale
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