White spot syndrome virus WSSV Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

Intended use:

This kit is suitable for the detection of Shrimp white spot syndrome virus (WSSV) in shrimp samples according to the different size or infection stage of different tissue samples, used for the auxiliary diagnosis of shrimp white spot syndrome virus (WSSV) infection.

Principle:

This kit uses a specific fragment of white spot syndrome virus (WSSV) to design a primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclization activity of Taq enzyme cuts the 5 '-terminal fluoromeric group from the probe, making it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of white spot syndrome virus (WSSV) was detected in the completely closed reaction system.

Biokey Aquaculture PCR testing solution.pdf

Main components:

Sample requirements:

1. Sample type

Tissue samples from prawn.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3.Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

4. Sample extraction&purification processing with professional machine below:

Using Micgene 242/244/244IVD and ABI QuantStudio5:

procedures are set as below:

FAM was selected for detection channel.

The results of interpretation

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

procedures are set as below:

FAM was selected for detection channel.

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 35 is considered as positive, and no Ct value or Ct value ≥35 is considered as negative.

Details please check from IFU!

We offer the full PCR testing solution to support the shrimp disease detection and monitoring on site.

Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team.

The company's core team has been in the field of IVD research and entrepreneurship for more than 10 years, and has mastered a wealth of molecular diagnostic POCT industrial application technology, SmartTRF immune detection technology, RT qPCR nucleic acid detection technology and independent research and development capabilities of instruments.

It has a number of independent core technologies in the fields of automation engineering, micro-precision manufacturing, immunofluorescence, nucleic acid detection and gene sequencing. Since its establishment, the company has applied for more than 60 patents and currently has 17 authorized patents. The company has two R&D centers for diagnostic reagents and instruments and equipment, totaling more than 500 square meters; it has established more than 2,000 square meters of GMP workshops and equipment production plants; it has SmartTRF new immune detection technology, nucleic acid detection technology (POCT) and three scientific services.