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Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized from wholesalers
     
    Buy cheap Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized from wholesalers
    • Buy cheap Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized from wholesalers
    • Buy cheap Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized from wholesalers

    Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized

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    Brand Name : Biokey
    Model Number : BIK-QL-H003S
    Certification : ISO 13485,CE
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized

    Real Time Diagnostics PCR Enterovirus 71 ( EV71 ) RT-PCR Detection Kit ( Lyophilized )


    Intended use:

    By using real time PCR systems, Enterovirus 71 real time PCR kit is used for the in vitro qualitative detection of Enterovirus 7. It can be used for the laboratory diagnosis and monitoring of the EV71 infection. The test results are only for clinical reference, not for case confirmation or exclusion.


    Test Principle:


    The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of EV71. The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of EV71 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

    Enterovirus 71 (EV71) RT-PCR Detection Kit (Lyophi...


    Product components:


    No.ComponentsBIK-QL-H003S
    1PCR Enzyme Mix (Lyophilized)48 tests/kit
    2EV71 Primer-Probe Mix/
    3Enzyme Mix Buffer (5×)/
    4Positive Control100 μL/tube
    5Negative Control100 μL/tube
    6RNase-free water1mL/tube
    7Paraffin oil1.5mL/tube


    Note: Do not mix the components from different batches for detection. The Positive Control of EV71 and internal control were constructed artificially, and they were not infectious.


    Compatible instruments:


    The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).


    Reaction Mix Preparation:


    Refer to one of the table below to prepare the reaction mix:


    1×volume required

    Resuspended master mix14 μL
    EV71 Primer & probe1 μL
    Total volume15 μL

    Multiply the numbers according to the number of tests.


    PCR amplification condition:


    PCR amplification according to the following procedure:


    StepCycleTemperatureTime
    1150℃10 min
    2194℃3 min

    3


    40

    94℃10 s
    60℃

    30 s

    (Collection of fluorescence)


    Select the detection channels as following:

    a) Select FAM channels to detect EV71.

    b) Select HEX/VIC channel to detect internal control.


    Reaction Mix Preparation:


    Refer to one of the table below to prepare the reaction mix:

    1×volume required
    Resuspended master mix14 μL
    EV71 Primer & probe1 μL
    Total volume15 μL

    Multiply the numbers according to the number of tests.


    Product picture:



    Operation details please check from IFU

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