Multiple Respiratory viral RT-PCR test kit (Lyophilized)
Intended use:
This kit is used for in vitro qualitative detection of
SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae from patients
with respiratory tract infection. The test results are for clinical
reference only, it cannot be used alone as the basis for the
diagnosis or exclusion of cases
Test Principle:
The kit adopt TaqMan fluorescent probe method. For the highly
conserved and specific region of SARS-CoV-2/Influenza
A+B/RSV/Adenovirus/M.pneumoniae, the primer probes, together with
RT-PCR reaction solutionfor fluorescent detection are designed and
labeled with different fluorescent groups. On the fluorescence
quantitative PCR instrument, the real-time fluorescence
quantitative PCR detection technology is used to realize the rapid
detection of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae
through the change of fluorescent signal.The detection system
contains positive internal control, used to monitor whether the
nucleic acid extraction process is normal.The kit adopt TaqMan
fluorescent probe method. For the highly conserved and specific
region of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae, the
primer probes, together with RT-PCR reaction solutionfor
fluorescent detection are designed and labeled with different
fluorescent groups. On the fluorescence quantitative PCR
instrument, the real-time fluorescence quantitative PCR detection
technology is used to realize the rapid detection of
SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae through the
change of fluorescent signal.The detection system contains positive
internal control, used to monitor whether the nucleic acid
extraction process is normal.
Biokey
Multiple Respiratory PCR testing solution.p...
Product components:
1. BIK-QL-H004:
| No. | Components | Amount |
| 1 | PCR Enzyme Mix (Lyophilized) | 96 tests/bottle |
| 2 | Primer-Probe Mix 1 (SARS-CoV-2/Influenza A+B ) | 50 μL/vial |
| 3 | Primer-Probe Mix 2 (RSV/Adenovirus/M.pneumoniae) | 50 μL/vial |
| 4 | Enzyme Mix Buffer (5×) | 400 μL/vial |
| 5 | Positive Control | 100 μL/tube |
| 6 | Negative Control | 100 μL/tube |
| 7 | RNase-free water | 1mL/tube |
| 8 | Paraffin oil | 1.5mL/tube*2 |
2. BIK-QL-H004S:
| No. | Components | Amount | | 1 | 1 (SARS-CoV-2/Influenza A+B Mix (Lyophilized)) | 24 tests/kit | | 2 | 2 (RSV/Adenovirus/M.pneumoniae Mix (Lyophilized)) | 24 tests/kit | | 3 | Positive Control | 100 μL/tube | | 4 | Negative Control | 100 μL/tube | | 5 | RNase-free water | 1mL/tube | | 6 | Paraffin oil | 1.5mL/tube |
|
Note: Do not mix the components from different batches for detection. The Positive Control of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae and internal control were constructed artificially, and they were not infectious.
Reagent preparatation:
(1) Preparation of [BIK-QL-H004] PCR mix as follows:
① Master Mix Preparation:Take out the components from the box and
let it thaw at room temperature until equilibrated. Resuspend the
Lyophilized Enzyme Mix in 400μL Enzyme Mix Buffer. Add 1mL
RNase-free water and gently pipette up and down. Avoid generating
air bubbles. Wash the wall of tube by pipetting to prevent
lyophilized powder from remaining. Place the tube aside for 30 min.
②Reaction Mix Preparation: Refer to the tables below to prepare the
reaction mix:
Ⅰ. SARS-CoV-2/Influenza A+B reaction mix:
| 1×volume required |
| Resuspended master mix | 14 μL |
| Primer-Probe Mix 1 | 1 μL |
| Total volume | 15 μL |
Ⅱ. RSV/Adenovirus/M.pneumoniae reaction mix:
| 1×volume required |
| Resuspended master mix | 14 μL |
| Primer-Probe Mix 2 | 1 μL |
| Total volume | 15 μL |
※Multiply the numbers according to the number of tests.
Lyophilization package :
Operation details please check from IFU
