Canine / Dog Helicobacter pylori ( Hp ) Bacteria DNA Detection Kit
( PCR-Fluorescent Probe )
Intend for Use:
This kit is suitable for the specific detection of pathogenic
Helicobacter pylori (Hp) in cat/dog feces samples.
Principle:
This kit selection of helicobacter pylori (Hp) specific fragment to
design primers probe probe, the probe can with primer amplification
area in the middle of a specific binding DNA template, in the
process of PCR extension, the circumscribed Taq polymerase enzymes
from the 5 'end of fluorescent groups on the probe will be cut
down, make it free in the reaction system, thereby quenching groups
out of the 3' end, Fluorescence was emitted and detected by
fluorescence PCR instrument to achieve the detection of
Helicobacter pylori nucleic acid (Hp) in the completely closed
reaction system.
Component:
| Composition | Quantity |
| PCR Liquid | 8 tube |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type
Cat/dog droppings.
2. Sample collection and transportation
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR
amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 94℃ | 3min |
| 2 | 45 | 94℃ | 10s |
| 60℃ | 20s collection fluorescent |
FAM was selected for detection channel
Positive judgment value or reference interval:
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 38＜Ct<45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all
positive.
2. Minimum detection limit: the detection limit of this kit is
10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch
precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | >0.980.
Product picture:
