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Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe from wholesalers
     
    Buy cheap Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe from wholesalers
    • Buy cheap Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe from wholesalers
    • Buy cheap Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe from wholesalers

    Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe

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    Brand Name : Biokey
    Model Number : Bik-Q-P039
    Certification : ISO13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : depending on order quantity
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    Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe

    Canine / Dog Helicobacter pylori ( Hp ) Bacteria DNA Detection Kit ( PCR-Fluorescent Probe )


    Intend for Use:

    This kit is suitable for the specific detection of pathogenic Helicobacter pylori (Hp) in cat/dog feces samples.

    Principle:

    This kit selection of helicobacter pylori (Hp) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to achieve the detection of Helicobacter pylori nucleic acid (Hp) in the completely closed reaction system.

    Component:


    CompositionQuantity
    PCR Liquid8 tube
    Positive control1 tube
    Negative control1 tube
    Note: components in different batches of kits should not be interchangeable.

    Sample requirements:


    1. Sample type

    Cat/dog droppings.

    2. Sample collection and transportation

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.


    PCR amplification and fluorescence detection (PCR detection area):


    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

    StepCycle numberTemperatureTime
    1194℃3min
    24594℃10s
    60℃20s collection fluorescent

    FAM was selected for detection channel


    Positive judgment value or reference interval:


    ResultsCt Value
    PositiveCt≤38
    NegativeNo Ct value or Ct value≥45
    Gray area38<Ct<45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    Product performance index:


    1. Accuracy

    The positive reference materials of testing enterprises were all positive.

    2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

    3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

    4. Linear, linear correlation coefficient | r | >0.980.


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