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RT PCR Enterovirus 71 Rapid Test Kit Lyophilized DNA Detection Kit

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Guangzhou BioKey Healthy Technology Co.Ltd

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RT PCR Enterovirus 71 Rapid Test Kit Lyophilized DNA Detection Kit

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Brand Name :	Biokey
Model Number :	BIK-QL-H003
Certification :	ISO 13485,CE
Price :	USD
Payment Terms :	L/C, T/T, Western Union
Supply Ability :	100,000 per day
Delivery Time :	Depending on order quantity

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RT PCR Enterovirus 71 Rapid Test Kit Lyophilized DNA Detection Kit

RT-PCR PCR assay diagnostic Enterovirus 71 ( EV71 ) DNA Detection Kit ( Lyophilized )

Intended use:

Adopting real time qPCR systems, Enterovirus 71 real time PCR kit is used for the in vitro qualitative detection of Enterovirus 7. It can be used for the laboratory diagnosis and monitoring of the EV71 infection. The test results are only for clinical reference, not for case confirmation or exclusion.

Test Principle:

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of EV71. The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of EV71 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

Product components:

No.	Components	BIK-QL-H003
1	PCR Enzyme Mix (Lyophilized)	96 tests/bottle
2	EV71 Primer-Probe Mix	100 μL/vial
3	Enzyme Mix Buffer (5×)	400 μL/vial
4	Positive Control	100 μL/tube
5	Negative Control	100 μL/tube
6	RNase-free water	1mL/tube
7	Paraffin oil	1.5mL/tube*2

Note: Do not mix the components from different batches for detection. The Positive Control of EV71 and internal control were constructed artificially, and they were not infectious.

Compatible instruments:

The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).

Reaction Mix Preparation:

Refer to one of the table below to prepare the reaction mix:

1×volume required
Resuspended master mix	14 μL
EV71 Primer & probe	1 μL
Total volume	15 μL

※Multiply the numbers according to the number of tests.

DATA ANALYSIS AND INTERPRETATION:

	Ct value	Result Analysis
1#	No Ct	negative
2#	≤38	Positive
3#	38~40	Re-test; if it is still 38~40, report as 2#

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Operation details please check from IFU

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