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Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized

Guangzhou BioKey Healthy Technology Co.Ltd
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    Buy cheap Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized from wholesalers
     
    Buy cheap Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized from wholesalers
    • Buy cheap Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized from wholesalers
    • Buy cheap Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized from wholesalers
    • Buy cheap Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized from wholesalers

    Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized

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    Brand Name : Biokey
    Model Number : BIK-QL-W051
    Certification : ISO 13485
    Price : USD
    Payment Terms : L/C, T/T, Western Union
    Supply Ability : 100,000 per day
    Delivery Time : Depending on order quantity
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    Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized

    Tilapia Lake Virus Nucleic Acid Detection Kit (Real time PCR, Lyophilized)



    Intended use:


    This kit is used for the specific detection of the Tilapia Lake virus (TiLV) in liver, spleen, kidney, and brain tissue samples of silk perch, Chinese mitten carp, Chinese giant salamander, and zebrafish.

    Principle:


    The kit synthesizes cDNA strands complementary to the RNA template using RNA as the template and primers as the starting point under the action of efficient reverse transcriptase. The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of Tilapia Lake Virus.


    The Real Time PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of Tilapia Lake Virus through the change of fluorescence signal.


    Main components:

    ComponentSpecification
    PCR reaction liquid24 tubes/kit
    Positive control100ul/tube
    Negative control100ul/tube
    reconstitution600ul/tube

    Note: components in different batches of kits should not be interchangeable.
    Product picture:


    Sample requirements:

    1. Sample type

    Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

    2. Transport

    Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

    3. Sample preservation

    It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.


    PCR amplification and fluorescence Detection (PCR detection area)

    Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


    StepCycle numberTemperatureTime
    1150°C20 min
    2195℃2min30s
    34595℃10s
    60℃30s collect fluorescent

    FAM is selected for detection channels.


    The results of interpretation:


    ResultsCt Value
    PositiveCt≤38
    NegativeNo Ct value or Ct value≥45
    Gray area38<Ct≤45

    Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


    Operation details please check from IFU!

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