Tilapia Lake Virus Nucleic Acid Detection Kit (Real time PCR,
Lyophilized)
Intended use:
This kit is used for the specific detection of the Tilapia Lake
virus (TiLV) in liver, spleen, kidney, and brain tissue samples of
silk perch, Chinese mitten carp, Chinese giant salamander, and
zebrafish.
Principle:
The kit synthesizes cDNA strands complementary to the RNA template
using RNA as the template and primers as the starting point under
the action of efficient reverse transcriptase. The specific primers
and specific fluorescent probes are designed for the nucleic acid
conserved region of Tilapia Lake Virus.
The Real Time PCR reaction solution and real-time fluorescent
quantitative PCR detection technology is applied on the
fluorescence quantitative PCR instrument for realizes the rapid
detection of Tilapia Lake Virus through the change of fluorescence
signal.
Main components:
| Component | Specification |
| PCR reaction liquid | 24 tubes/kit |
| Positive control | 100ul/tube |
| Negative control | 100ul/tube |
| reconstitution | 600ul/tube |
Note: components in different batches of kits should not be
interchangeable.
Product picture:
Sample requirements:
1. Sample type
Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic
products, etc.
2. Transport
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -20℃.
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR
amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 50°C | 20 min |
| 2 | 1 | 95℃ | 2min30s |
| 3 | 45 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
FAM is selected for detection channels.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 38＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
Operation details please check from IFU!
